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| Espinosa,Ivette; Báez,M; Percedo,María Irian; Martínez,Siomara. |
| Streptococcus suis is a gram-positive bacterium that causes serious diseases in pigs and in humans with occupational risk. The DNA extraction methods for amplification of gene fragments by PCR for typing S.suis may be complex, and expensive chemical reagents and time consuming. The aim of this study was to evaluate a method for the rapid release of the genomic DNA from S.suis colonies by using a physical method based on heating and freezing; in this case, temperatures of 100ºC and 95ºC were tested. The results showed that DNA extraction directly from colonies by heating at 100ºC could be useful for an easy genotyping of S.suis strains in a short time, while 95ºC was not sufficient for DNA release. The detection limit of the PCR assay using DNA obtained by... |
| Tipo: Journal article |
Palavras-chave: Streptococcus suis; Direct colony PCR. |
| Ano: 2013 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000100009 |
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| Espinosa,Ivette; Domínguez,Patricia; Lobo,Evelyn; Alfonso,P; Martínez,Siomara. |
| Streptococcus suis es un coco anaerobio facultativo, considerado un importante agente asociado a meningitis, artritis, endocarditis y neumonía en cerdos. En este trabajo se realizó el aislamiento de S.suis a partir de diferentes condiciones clínicas como neumonía, abscesos en articulaciones y tonsilas de animales asintomáticos. Se identificaron 14 cepas de S. suis: 11 a partir de pulmones, 2 de abscesos y 1 de tonsila. Las cepas mostraron diferentes patrones de pruebas bioquímicas. El gen que codifica para la hemolisina denominados suilisina (syl) se detectó por PCR solamente en 5 cepas de las cuales 4 proceden de pulmón y una de tonsila. Sin embargo, en los aislamientos procedentes de abscesos y asociados a artritis no se detectó la presencia de este gen,... |
| Tipo: Journal article |
Palavras-chave: Streptococcus suis; PCR; Suilisina; Cerdos; Streptococcu suis; PCR; Suilysine; Pigs. |
| Ano: 2010 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2010000100007 |
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| Espinosa,Ivette; Áreas,Michel; Percedo,María Irian; Lobo,Evelyn; Martínez,Siomara; Gottschalk,Marcelo. |
| Streptococcus suis is an important swine pathogen in most countries with intensive swine production. S. suis has also been isolated in humans and other animal species. Data regarding serotyping of S. suis isolates from diseased pigs in Cuba using a reference antiserum are not available. S. suis isolates from pigs of four farms located in the western region of Cuba were characterized by serotyping. Most of the isolates was collected from pigs with pneumonia, followed by arthritis cases. Serotypes 2, 4, 8, 15, 16 and 34 were found in isolates from diseased pigs during the period 2002-2008, while serotypes 1, ½, 2, 3, 9, 13, 23, 24 and 31were found from samples collected during 2009-2014. Serotype 2 recovered from systemic (arthritis) and localized... |
| Tipo: Journal article |
Palavras-chave: Swine; Streptococcus suis; Serotype. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000300009 |
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| Del'Arco,A.E.; Santos,J.L.; Bevilacqua,P.D.; Faria,J.E.; Guimarães,W.V.. |
| The epidemic aspects of swine infections caused by Streptococcus suis were studied, focusing mainly on the occurrence of several serotypes. A total of 323 samples of S. suis were isolated from clinically ill animals, serotyped according to the co-agglutination procedure, and analyzed. The serotyping revealed that S. suis was present in several Brazilian states. The largest number was isolated from the states of Minas Gerais (62.5%), São Paulo (10.8%), and Paraná (9.3%). Serotype 2 was the most frequent (61.0%), followed by the serotypes 1, 3, 4, 7, and 8. The largest number of isolations was obtained from the brain (60.1%), followed by the lungs (10.4%). About 9.4% of the cases were due to septicemia. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig; Streptococcus suis; Serotypes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400016 |
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